Goat anti-Mouse IgG (H+L) Secondary Antibody, Biotin/山羊抗小鼠IgG（H + L）二抗，Biotin

货号：31800

规格：2 mL

价格：2319

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	1.3 mg/mL	用法：	1:20,000-1:400,000(ELISA)；1:200-1:1,000(Flow)；1:500-1:5,000(ICC)；1:500-1:5,000(IF)；1:500-1:5,000(IHC)；1:500-1:5,000(IP)；1:5,000-1:20,000(WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31800 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31800 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Reconstitute with 1.5 mL of distilled water.Country of Origin: USA靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (31800) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Product # 31800) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Journal of neuroimmune pharmacology Multimodal analysis in acute and chronic experimental autoimmune encephalomyelitis.

技术参数

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