Nanog Monoclonal Antibody (23D2-3C6), DyLight 650

货号： MA1-017-D650

规格：100 µL

价格：4070

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Nanog

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：23D2-3C6

荧光染料：DyLight 650

类型:	单抗	同型对照：	IgG1
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:10-1:250(ICC);1:10-1:250(IF)

Product Specific InformationMA1-017-D650 has been successfully used in ICC/IF applications with human samples.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (MA1-017-D650) in IFImmunofluorescent analysis of Nanog (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated Nanog monoclonal antibody (Product # MA1-017-D650) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification. Nanog Antibody (MA1-017-D650) in IFImmunofluorescence analysis of Nanog was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nanog Mouse Monoclonal Antibody (Product # MA1-017D-650) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: red). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: green) was stained with Alexa Fluor™ 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows Nanog negative cell line HeLa with no signal. Panel f represents control cells with Isotype control to assess background. The images were captured at 60X magnification. Nanog Antibody (MA1-017-D650)Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis showed expression of Nanog in NTERA-2 and not in HeLa which is a negative model for Nanog using Nanog Monoclonal Antibody (Product # MA1-017D-650).

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