Nanog Monoclonal Antibody (23D2-3C6), HRP

货号： MA1-017-HRP

规格：100 µL

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

抗原：Nanog

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：23D2-3C6

荧光染料：DyLight 650

类型:	单抗	同型对照：	IgG1
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:250-1:1000L(WB)

Product Specific InformationWestern blot analysis of MA1-017-HRP detects an ~38 kDa protein in embryonal carcinoma cells. MA1-017-HRP shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (MA1-017-HRP) in WBWestern blot analysis of Nanog was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Nanog monoclonal antibody (Product # MA1-017-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17874.htm