SSEA1 Monoclonal Antibody (MC-480)

货号： MA1-022,MA1-022X

规格：100ug,20uL

价格：4070,2148

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SSEA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：MC-480

荧光染料：其它

类型:	单抗	同型对照：	IgM
免疫原性：	BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).	用法：	1:100(Flow);1:50-1:500(ICC);1:50-1:500(IF);1:50(WB)

Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA1 Antibody (MA1-022) in IFImmunofluorescent analysis of SSEA-1 using anti-SSEA-1 monoclonal antibody (Product # MA1-022) shows specific expression in mouse testicular teratoma F9 cells (shown in green) but not in negative control human NTERA-2 teratocarcinoma cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SSEA-1 (Product # MA1-022), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a fluorescent dye labeled goat-anti-mouse IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. SSEA1 Antibody (MA1-022) in IFImmunofluorescent analysis of SSEA1 (red) in mouse embryonic CJ7 stem cells grown on 0.1% gelatin. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with 10% BSA in PBS for 30 min at 37°C. Cells were stained with a SSEA1 monoclonal antibody (Product # MA1-022) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C and then incubated with a RRX-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Note: Data courtesy of Innovators program. SSEA1 Antibody (MA1-022) in FlowFlow cytometry analysis of SSEA-1 (blue histogram) on H9 human embryonic stem cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a SSEA-1 monoclonal antibody (Product # MA1-022) or an IgM isotype control (red histogram) at a 1:100 dilution for 1 hour on ice. For flow analysis, a 30-minute incubation with fluorescein conjugated secondary antibody was performed and 100,000 cells were acquired for each sample.

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参考文献：

1.Frontiers in molecular neuroscience Comparison ofin vitroNeuronal Differentiation Capacity Between Mouse Epiblast Stem Cells Derived From Nuclear Transfer and Naturally Fertilized Embryos.

技术参数

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