Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody/山羊抗小鼠IgG (H+L)Superclonal™重组二抗

货号：A28174

规格：1 mg

价格：1826

产品类型：二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.01-0.5 µg/mL（ELISA）；0.1-2 µg/mL (ICC)；0.1-2 µg/mL (IF); 0.05-1 µg/mL(WB)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (A28174) in IFRepresentative images demonstrating lot-to-lot consistency of performance across three lots of Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A28174) in immunocytochemistry. No primary control to assess background is shown. Endogenous Cytochrome-C (A) and endogenous Nucleostemin (B) in U20S human osteocarcinoma cells was labeled (green) with A) Mouse anti-Cytochrome C and B) Mouse anti-Nucleostemin primary antibody, Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A28174) (0.4 µg/mL, 1:2500) and visualized using Alexa Fluor® 488 Rabbit anti-Goat IgG (H+L) as tertiary antibody (Product # A-11078) (1:400). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). F-actin (red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381) (1:200). Mouse IgG (H+L) Secondary Antibody (A28174) in IF Representative images demonstrating lot-to-lot consistency of performance across three lots of Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A28174) in immunocytochemistry. No primary control to assess background is shown. Endogenous Cytochrome-C (A) and endogenous Nucleostemin (B) in U20S human osteocarcinoma cells was labeled (green) with A) Mouse anti-Cytochrome C and B) Mouse anti-Nucleostemin primary antibody, Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (Product # A28174) (0.4 µg/mL, 1:2500) and visualized using Alexa Fluor® 488 Rabbit anti-Goat IgG (H+L) as tertiary antibody (Product # A-11078) (1:400). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). F-actin (red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381) (1:200). Mouse IgG (H+L) Secondary Antibody (A28174) in WBEndogenous alpha-tubulin (A) and beta-actin (B) was detected at 52kDA and 46 KDa using Mouse anti-alpha -tubulin (Product # 32-2500) and beta-actin (Product # AM4302) primary antibody and Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Unconjugated (Product # A28174) (0.4 µg/mL, 1:2500). Rabbit anti-Goat IgG (H+L) Superclonal secondary antibody, HRP conjugate as the tertiary Antibody (Product # A27014) (1:3000).No primary control to assess background (C) and loading control (LC) mouse anti-GAPDH (Product # 39-8600) is shown. Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa human cervical carcinoma cells (lane 1-5) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. Chemiluminescence was detected using the Pierce™ ECL Western Blotting Substrate (Product # 32106).

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