SSEA1 Monoclonal Antibody (MC-480), DyLight 488

货号：MA1-022-D488

规格：100 µL

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

抗原：SSEA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：MC-480

荧光染料：DyLight 488

类型:	单抗	同型对照：	IgM
免疫原性：	BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).	用法：	1:50-1:500(Flow);1:20-1:200(ICC);1:20-1:200(IF)

Product Specific InformationMA1-022-D488 has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA1 Antibody (MA1-022-D488) in IFImmunofluorescent analysis of SSEA-1 (green) showing membrane staining of F9 cells (right panel) compared to negative undifferentiated NCCIT control cells (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.SSEA1 Antibody (MA1-022-D488) in FlowFlow cytometry analysis of SSEA-1 in F9 (green filled histogram) and NCCIT cells (green unfilled histogram) compared to unstained cells (blue histogram). Positive staining is observed on F9 cells when compared to no antibody control (middle panel) and to SSEA-1-negative undifferentiated NCCIT cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature, and incubated with a DyLight 488-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-D488) in 2% BSA-PBS at a dilution of 1:100 for 60 minutes at room temperature. Cells were washed and re-suspended in PBS for FACS analysis. SSEA1 Antibody (MA1-022-D488) in IFImmunofluorescent analysis of SSEA1 (green) in mouse embryonic CJ7 stem cells grown on 0.1% gelatin. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a DyLight 488 conjugated SSEA1 monoclonal antibody (Product # MA1-022-D488) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C. Nucleus DNA (blue) was stained with DAPI (Product # D1306).

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