c-Myc Monoclonal Antibody (9E10)

货号： 13-2500

规格：200 µg

价格：3990

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：c-Myc

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：9E10

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	A 32 amino acid synthetic peptide (aa 408-439) derived from the C-terminus of the human c-myc protein.	用法：	0.1-1.0 µg/mL(ELISA);2-10 µg/mL(IF);1 -2µg/mL(WB);1:100(IHC(P));2-5 µg(IP)

Target InformationThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

c-Myc Antibody (13-2500) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HEK 293 (Lane 2), A-375 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), U-87MG (Lane 6) and K562 (Lane 7). The blots were probed with Anti-c-Myc Epitope Tag Mouse Monoclonal Antibody (Product # 13-2500, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 50 kDa band corresponding to c-Myc was observed across cell lines tested except U-87 MG. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). c-Myc Antibody (13-2500) in IHC (P)Immunohistochemistry analysis of c-Myc showing staining in the nucleus of paraffin-embedded human lung squamous carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- c-Myc Monoclonal Antibody (9E10) (Product # 13-2500) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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参考文献：

1.Nature communications A biosensor-based framework to measure latent proteostasis capacity. "13-2500 was used in Western Blotting to reveal that HSP70 chaperone cycle is rate limited by HSP70 holdase activity under normal conditions, but can be overcome by increased levels of BAG1 nucleotide exchange factor to HSPA1A or activation of the heat shock gene cluster." AuthorsWood RJ,Ormsby AR,Radwan M,Cox D,Sharma A,Vöpel T,Ebbinghaus S,Oliveberg M,Reid GE,Dickson A,Hatters DM 2.Scientific reports Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease. "132500 was used in western blot to analyze the expression and characteristics of the secreted metalloprotease ADAMTS17 in regards to fibrillin microfibrils" AuthorsHubmacher D,Schneider M,Berardinelli SJ,Takeuchi H,Willard B,Reinhardt DP,Haltiwanger RS,Apte SS 3.The Journal of biological chemistry Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion. "132500 was used in western blot to report that mammalian actin-binding protein-1 and FHL2 are binding partners that regulate Rho GTPase signaling and breast cancer cell invasion" AuthorsBoateng LR,Bennin D,De Oliveira S,Huttenlocher A 4.Annals of allergy, asthma and immunology : official publication of the American College of Allergy, Asthma, and Immunology The NPC2 protein: A novel dog allergen. "132500 was used in western blot to discuss dog allergens such as Can f 1" AuthorsKhurana T,Newman-Lindsay S,Young PR,Slater JE 5.The Journal of general virology The levels of epithelial anchor proteins β-catenin and zona occludens-1 are altered by E7 of human papillomaviruses 5 and 8. "132500 was used in western blot to show that beta-catenin and ZO-1 are targets of E7 of the oncogenic human papillomaviruses types 5 and 8" AuthorsHeuser S,Hufbauer M,Marx B,Tok A,Majewski S,Pfister H,Akgül B

技术参数

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