数据：

U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green) and cultured overnight. The following day cells were treated with 60uM Chloroquine and cultured overnight. Cells were then counterstained with 1ug/mL Hoeschst 33342 (blue) and imaged on a Delta Vision wide field microscope.

Monitoring autophagy, tubulin and mitochondria in U-2 OS cells

U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (pink) and CellLight® Tubulin-GFP (cyan) and cultured overnight. The following day cells were treated with 60uM Chloroquine and cultured overnight. Cells were then counterstained with 50nM MitoTracker® Deep Red (orange) for 15 minutes at 37C washed in dye free media and labeled with 1ug/mL Hoeschst 33342 (red). Cells were imaged on a Zeiss LSM 710 confocal microscope.

Labeling autlysosomes with LysoTracker Deep Red.

A549 cells were transduced Premo™ Autophagy Sensor LC3B-RFP (green) and cultured for 24hours. Chloroquine at a final concentration of 60uM was added and the cells were cultured for a further 16 hours. A549 cells were labeled with 50nM LysoTracker® Deep Red (red) and Hoechst 33342 (blue) and imaged using standard DAPI/TRITC & Cy5 filter sets.

相关产品：

▪Premo™ Autophagy Sensor RFP-p62 Kit（货号#P36241）

▪Premo™ Autophagy Sensor GFP-p62 Kit（货号#P36240）

▪Premo™ Autophagy Sensor GFP-LC3B Kit（货号#P36235）

▪Premo™ Autophagy Sensor RFP-LC3B Kit（货号#P36236）

▪Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit（货号#P36239）