TAMRA Super Column (5'-DMT-mdC(TEG-TAMRA)-Phos-CPG); 500 Å
货号：SCG5-5008-1,SCG5-5008-2
规格：1 µmol,200 nmol
价格：1055.25,281.4
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Biosearch Technologies' 5'-DMT-mdC(TEG-TAMRA)-Phosphate CPG is based on a modified cytosine residue linked to the TAMRA moiety via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via a phosphate linkage. Our TAMRA CPG supports allow for the introduction of a reporter or quencher molecule onto the 3'-terminus end of the oligonucleotide. |
| 特性： |
| ▪ Formula:C77H91N8O21PS▪ Molecular Weight:1527.63▪ Appearance:pink powder▪ Absorption Maximum (Lambda Max):555▪ Extinction Coefficient at Lambda max:90000▪ Extinction Coefficient at 260 nm:31980▪ Fluorescence Maximum:576 |
| 产品用途： |
| Synthesis conditions:Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted.Cleavage conditions:Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia or methylamine must be avoided, as it will cause degradation of the TAMRA moiety.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60° C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60°C.ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended:
1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.
2) An additional oxidation step.
3) OPTIONAL (but may help): extension of the coupling time to 20 sec. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：998.91 |
| 数据： |
|
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-21046.htm
QQ:1749072012