OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience™

货号：12-5841-82,12-5841-80

规格：100 ug,25 ug

价格：3402,1545

产品类型：流式抗体

品牌：eBioscience

抗原：OCT3/4

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：EM92

荧光染料：PE

类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.5µg/mL(Flow)

Product Specific InformationDescription: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).Applications Reported: This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of F9 embryonal carcinoma cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationPOU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

OCT3/4 Antibody (12-5841-82) in FlowStaining of F9 embryonal carcinoma cell line with 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.25 µg of Anti-Mouse OCT3/4 PE (filled histogram). Staining was carried out using the Foxp3 Staining Buffer Set (00-5523). Total cells were used for analysis.

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参考文献：

1.Genome researchThe HUSH complex cooperates with TRIM28 to repress young retrotransposons and new genes."12-5841 was used in Flow cytometry/Cell sorting to study the role of the human silencing hub complex in naive pluripotent stem cells."AuthorsRobbez-Masson L,Tie CHC,Conde L,Tunbak H,Husovsky C,Tchasovnikarova IA,Timms RT,Herrero J,Lehner PJ,Rowe HM2.PloS oneHighly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.

技术参数

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