SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eBioscience™

货号：14-8843-80

规格：25 µg

价格：1514

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA4

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：eBioMC-813-70 (MC-813-70)

荧光染料：Purified

类型:	单抗	同型对照：	IgG3
免疫原性：		用法：	1 µg/test(Flow)

Product Specific InformationDescription: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA4 Antibody (14-8843-80) in FlowStaining of the 2102Ep cell line with 0.5 µg of Mouse IgG3 Isotype Control Purified (Product # 14-4742-82) (open histogram) or 0.5 µg of Anti-Human SSEA-4 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012).Total viable cells were used for analysis.

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参考文献：

1.Stem cells and developmentSimplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.2.The Journal of biological chemistrySialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells."14-8843 was used in Flow cytometry/Cell sorting to characterise acid glycosphingolipids isolated from human embryonic stem cells, revealing a novel marker of undifferentiated cells, sialyl-lactotetra."AuthorsBarone A,Säljö K,Benktander J,Blomqvist M,Månsson JE,Johansson BR,Mölne J,Aspegren A,Björquist P,Breimer ME,Teneberg S3.BiomicrofluidicsLectin-functionalized microchannels for characterizing pluripotent cells and early differentiation."14-8843 was used in Flow cytometry/Cell sorting to track the differentiation of embryonic stem cells into the three embryonic germ layers; endoderm, mesoderm, and ectoderm, using surface lectins."AuthorsVickers DA,Kulik M,Hincapie M,Hancock WS,Dalton S,Murthy SK

技术参数

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