SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 570, eBioscience™
货号：41-8843-82,41-8843-80
规格：100 ug,25 ug
价格：4189,1892
产品类型：流式抗体
品牌：eBioscience
物种：人/小鼠
宿主：小鼠
抗体亚型：其它
荧光染料：其它
类型: | 单抗 | 同型对照： | IgG3 |
免疫原性： | | 用法： | 10 µg/mL(ICC);10 µg/mL(IF) |
Product Specific InformationDescription: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in immunocytochemistry. This product has not been validated for flow cytometric analysis.Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by immunocytochemistry on fixed NTERA-2 and 2102Ep cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nmTarget InformationSSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| SSEA4 Antibody (41-8843-82) in ICCImmunocytochemistry on fixed NTERA-2 cells using 10 µg/mL Anti-Human SSEA-4 eFluor® 570. Nuclei are stained with DAPI. |
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