TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), eBioscience™

货号：14-8863-82

规格：100 µg

价格：2916

产品类型：流式抗体

品牌：eBioscience

抗原：TRA-1-60

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-60

荧光染料：Purified

类型:	单抗	同型对照：	IgM
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

Product Specific InformationDescription: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-60 (Podocalyxin) Antibody (14-8863-82) in FlowStaining of 2102Ep human embryonal carcinoma cell line with 0.25 µg of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (open histogram) or 0.25 µg of Anti-Human TRA-1-60 Purified (filled histogram) followed by Anti-Mouse IgM PE (Product # 12-5890-82).Total viable cells were used for analysis.

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参考文献：

1.Journal of the American Heart AssociationToward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome."14-8863 was used in Flow cytometry/Cell sorting to evaluate the use of cardiomyocytes differentiated from urine-derived stem cells as a new cellular model for studying patients' specific arrhythmia mechanisms."AuthorsJouni M,Si-Tayeb K,Es-Salah-Lamoureux Z,Latypova X,Champon B,Caillaud A,Rungoat A,Charpentier F,Loussouarn G,Baró I,Zibara K,Lemarchand P,Gaborit N2.Stem cell reviewsPRMT5 is required for human embryonic stem cell proliferation but not pluripotency.3.Orphanet journal of rare diseasesInduced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation."14-8863 was used in Immunocytochemistry to investigate the cellular mechanisms behind fibrodysplasia ossificans progressiva using a novel human induced pluripotent stem cell disease model."AuthorsMatsumoto Y,Hayashi Y,Schlieve CR,Ikeya M,Kim H,Nguyen TD,Sami S,Baba S,Barruet E,Nasu A,Asaka I,Otsuka T,Yamanaka S,Conklin BR,Toguchida J,Hsiao EC4.The Journal of biological chemistrySialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells."14-8863 was used in Immunohistochemistry to characterise acid glycosphingolipids isolated from human embryonic stem cells, revealing a novel marker of undifferentiated cells, sialyl-lactotetra."AuthorsBarone A,Säljö K,Benktander J,Blomqvist M,Månsson JE,Johansson BR,Mölne J,Aspegren A,Björquist P,Breimer ME,Teneberg S5.Oncology lettersDimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas."14-8863 was used in Western Blotting to demonstrate that dimethyl sulfoxide perturbs differentiation, reduces stemness and induces resistance to cisplatin in human embryonal carcinoma cells."AuthorsKita H,Okamoto K,Kushima R,Kawauchi A,Chano T

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