Nanog Monoclonal Antibody (hNanog.2), eBioscience™

货号：14-5768-82,14-5768-80

规格：100 µg,25 µg

价格：4291,2266

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：其它

荧光染料：Purified

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	5.0 µg/mL(ICC);5 µg/mL(IF);1 µg/mL(WB)

Product Specific InformationDescription: The hNanog.2 antibody recognizes human Nanog, a homeobox transcription factor expressed in embryonic stem cells (ESC). Nanog is essential for self-renewal of ESC and the inner cell mass and is throught to function in the same capacity as other factors such as Oct4 and Sox2. Nanog plays a crucial role in embryonic cell fate specification following formation of the blastocyst by regulating proliferation and preventing differentiation. Nanog functions by directly binding DNA consensus sequences to activate or repress transcription. In differentiating ESC, Nanog binds to its own promoter leading to repression of expression. Nanog has been reported to be overexpressed in germ cell tumors of the gonads and central nervous system as well as breast and colorectal cancer.Applications Reported: This hNanog.2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates and can be used at less than or equal to 1 µg/mL. The hNanog.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. The hNanog.2 antibody has been tested by immunocytochemistry on formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (14-5768-82) in ICCImmunocytochemistry of formaldehyde-fixed and permeabilized N-Tera cells using 5 µg/mL Anti-Human Nanog Purified, followed by 10 µg/mL F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI, colocalization appears pink. Nanog Antibody (14-5768-82) in WBKnockdown of Nanog was achieved by transfecting NTERA-2 cells with Nanog specific siRNAs (Silencer® select Product # s36650). Western blot analysis (Fig. a) was performed using whole cell extracts from the NTERA-2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and un-transfected cells (lane 1). The blots were probed with Antibody (Product # 14-5768-82, 1µg/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometry analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Nanog. Nanog Antibody (14-5768-82) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma using 20 µg/mL Anti-Human Nanog Purified, followed by Anti-Mouse IgG Biotin, Avidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

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参考文献：

1.Cell discoveryNANOG reprograms prostate cancer cells to castration resistance via dynamically repressing and engaging the AR/FOXA1 signaling axis.2.PloS oneNanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species.3.PloS oneCharacterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency.4.BMC genomicsA mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis."14-5768 was used in Immunocytochemistry to characterise the genes involved in bovine pluripotency."AuthorsBrinkhof B,van Tol HT,Groot Koerkamp MJ,Riemers FM,IJzer SG,Mashayekhi K,Haagsman HP,Roelen BA

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