Nanog Monoclonal Antibody (1E6C4)

货号：MA5-15469

规格：100 µL

价格：5840

产品类型：流式抗体

品牌：Thermo Fisher

抗原：Nanog

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：1E6C4

荧光染料：Purified

类型:	单抗	同型对照：	IgG1
免疫原性：	Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.	用法：	5 µg/mL(ICC);5 µg/mL(IF);1/500 - 1/2000(WB)

Product Specific InformationMA5-15469 targets NANOG in IF and WB applications and shows reactivity with Human samples.The MA5-15469 immunogen is purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.MA5-15469 detects NANOG which has a predicted molecular weight of approximately 35kDa.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (MA5-15469) in IFImmunofluorescence analysis of Nanog was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nanog Monoclonal Antibody (Product # MA5-15469) at 5µg/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows Nanog negative cell line HeLa with no signal. Panel f represents control cells with no primary to assess background. The images were captured at 60X magnification. Nanog Antibody (MA5-15469) in IFImmunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using NANOG monoclonal antibody (Product # MA5-15469) (Green). Red: Actin filaments have been labeled with DY-554 phalloidin.

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