Nanog Polyclonal Antibody

货号：PA5-85110

规格：100 µL

价格：4806

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：	IgG
免疫原性：	Recombinant protein encompassing a sequence within the center region of human NANOG.	用法：	1:1000-1:10000(ELISA);1:100-1:1000(ICC);1:100-1:1000(IF);1:500-1:3000(WB);1:100-1:500(IP)

Product Specific InformationKeep as concentrated solution.Positive Control: HeLa nucleus, NIH-3T3, mouse ESC.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (PA5-85110) in IPImmunoprecipitation analysis of Nanog in NT2D1 whole cell extract with Nanog polyclonal antibody (Product # PA5-85110) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody. Nanog Antibody (PA5-85110) in IFImmunocytochemistry analysis of Nanog in NT2D1 cells using Nanog polyclonal antibody (Product # PA5-85110) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody. Nanog Antibody (PA5-85110) in ICCImmunocytochemistry analysis of Nanog in 4% paraformaldehyde-fixed human embryonic stem cells using Nanog polyclonal antibody (Product # PA5-85110) at a dilution of 1:400. Nanog Antibody (PA5-85110) in ELISAELISA analysis of Nanog using Nanog polyclonal antibody (Product # PA5-85110) at a dilution of 5 µg/mL.

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