Nanog Polyclonal Antibody

货号：PA5-47376

规格：100 µg

价格：4880

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor plus 680

类型:	多抗	同型对照：	IgG
免疫原性：	E. coli-derived recombinant mouse Nanog Trp154-Leu262	用法：	0.25 µg/1x10^6 cells（Flow);5-15 µg/mL(ICC);5-15 µg/mL(IF)

Product Specific InformationThis antibody detects recombinant mouse Nanog in Western blots. Approximately 50% cross-reactivity with recombinant human Nanog is observed.Reconstitute at 0.2 mg/mL in sterile PBS.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (PA5-47376) in FlowFlow cytometric analysis of D3 mouse embryonic stem cell line was stained with Goat Anti-mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47376) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.

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