产品优点 - Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.- Generate clones with inducible copy number using CopyControl™ vectors.- Achieve high transformation efficiencies: >1 × 10^10 cfu/µg pUC19 DNA.- Increase Gibson Assembly® colony counts with high-efficiency electroporation- 克隆大插入片段并转化大质粒-至少145 kb质粒DNA。- 使用CopyControl™向量生成具有可诱导拷贝数的克隆。- 实现高转化效率：> 1×10^10 cfu / µg pUC19 DNA。- 通过高效电穿孔增加GibsonAssembly®菌落数。

产品应用 - Mutant trfA gene under tightly regulated control of an inducible promoter for complete copy number control of CopyControl clones. - Supports blue/white screening of vectors expressing the LacZ′ α-complementing peptide. - Restriction minus for efficient cloning of methylated genomic (e.g. mammalian genomic) DNA.- Endonuclease minus (endA1) to ensure high yields of clones. - Recombination minus (recA1) to ensure the stability of large cloned inserts.- 严格控制可诱导启动子控制的突变体trfA基因，可完全控制CopyControl克隆的拷贝数。- 支持对表达LacZ'α互补肽的载体进行蓝/白筛选。- 有效克隆甲基化基因组（例如哺乳动物基因组）DNA的限制性酶减。- 核酸内切酶负（endA1）可确保高产量的克隆。- 重组减去（recA1）可确保大克隆插入片段的稳定性。