SOX2 Monoclonal Antibody (10F10)

货号：MA5-15734

规格：100 µL

价格：5463

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：SOX2

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：10F10

荧光染料：其它

类型:	单抗	同型对照：	IgG1
免疫原性：	Purified recombinant fragment of human SOX2 expressed in E. Coli.	用法：	1:200-1:1000(ICC);1:200-1:1000(IF);1:200-1:1000(IHC);1/500 - 1/2000(WB)

Product Specific InformationMA5-15734 targets SOX2 in IF, IHC, and WB applications and shows reactivity with Human samples.The MA5-15734 immunogen is purified recombinant fragment of human SOX2 expressed in E. Coli.MA5-15734 detects SOX2 which has a predicted molecular weight of approximately 34kDa.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (MA5-15734) in ChIPEnrichment of endogenous SOX2 protein at specific gene loci using Anti-SOX2 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-SOX2 Mouse Monoclonal Antibody (Product # MA5-15734, 8 ul) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over OCT4 promoter (active) and SAT2 satellite repeats and SAT alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. SOX2 Antibody (MA5-15734) in IFImmunofluorescence analysis of NTERA-2 cells using SOX2 monoclonal antibody (Product # MA5-15734) (Green). Red: actin filaments have been labeled with phalloidin. SOX2 Antibody (MA5-15734) in IFImmunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # MA5-15734) at 1:250 dilution in 0.1% BSA,incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows SOX2 negative cell line HeLa with no signal. Panel f represents control cells with no primary control to assess background. The images were captured at 60X magnification. SOX2 Antibody (MA5-15734) in IHCImmunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) using SOX2 monoclonal antibody (Product # MA5-15734) followed with DAB staining.

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