Rat anti Dog CD8 antibody, clone YCATE55.9
货号：MCA1039A647
规格：100 Tests/1ml
价格：4280
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD8
物种：人
宿主：大鼠
抗体亚型：IgG1
克隆号：YCATE55.9
荧光染料：Alexa Fluor 700
| 产品介绍 | | |
| Rat anti Dog CD8 antibody, clone YCATE55.9was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobboldet al.1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α (Gormanet al.1994) and blocks MHC class I dependant T-cell responsesin vitroandin vivo.Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogsin vivo. (Watsonet al.1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Billeret al.2010). |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Canine CD8 alpha chimaeric human IgG1 Fc fusion protein. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Fusion Partners | Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | 1/100 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Gorman, S.D.et al.(1994) Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens.Tissue Antigens. 43 (3): 184-8.3.Watson, C.J.et al.(1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.Br J Surg. 80 (11): 1389-92. |
技术参数 产品应用 flow
产品类型 单克隆抗体
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