CopyControl™ Fosmid Autoinduction Solution, 50 mL（停产））
货号：AIS107F
规格：50 mL
价格：1234
产品类型：质粒
品牌：Lucigen
| The CopyControl™ Fosmid Autoinduction Solution is designed to induce CopyControl Fosmid clones and clones retrofitted with the EZ-Tn5™ <oriV/KAN-2> Transposon, grown in TransforMax™ EPI300™ E. coli cells, from single-copy number to a higher-copy number of approximately 50 fosmids per cell. The Fosmid Autoinduction Solution induces expression of a mutant trfA gene contained in the TransforMax EPI300 cells. Expression of trfA gene results in initiation of replication from the oriV high copy origin of replication and subsequent amplification of the CopyControl clones to high copy number. The Fosmid Autoinduction protocol improves upon the existing induction protocol by including the autoinduction supplement in the media prior to culture inoculation, removing the need for time-consuming subculturing and the 2-hour incubation required in the standard induction protocol. The Fosmid Autoinduction Solution also contains cell growth enhancers which boost cell numbers and typically provides higher DNA yields than with the standard CopyControl induction protocol. The hands-off autoinduction protocol makes CopyControl Fosmid Autoinduction solution ideal for high-throughput purification protocols in 96-well format. The autoinduction solution is also compatible with larger scale DNA purifications and can be scaled according to the amount of media used. |
| 优点： |
| ▪Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts ▪ Stabilize clones with copy number control▪Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit |
| 相关产品： |
| ▪CopyControl™ Fosmid Library Production Kit（货号#CCFOS110）▪ CopyControl™ HTP Fosmid Library Production Kit（货号#CCFOS059）▪CopyControl™ Induction Solution（货号#CCIS125） |
技术参数 产品优点 - Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts
- Stabilize clones with copy number control
- Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit
产品应用 - Preparation of complete and unbiased fosmid libraries, from any biological sample, that can be maintained at single-copy number and induced to high-copy number as needed, using the CopyControl™ Autoinduction Solution.
- Construction of metagenomic libraries from microbes present in environmental samples such as water and soil.
推荐用于 Efficient and improved systems for constructing libraries of approximately 40 kb clones in both standard or high-throughput screening versions.
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