Rat anti-Mouse IgG1 Secondary Antibody, APC, eBioscience™/大鼠抗小鼠IgG1二抗, APC, eBioscience™

货号：17-4015-82 ,17-4015-80

规格：100 μg,25 μg

价格：1517,565

产品类型：细胞分选

品牌：美国精骐

抗体类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）； Assay-Dependent (IHC(F))

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (17-4015-82) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (blue histogram) or Anti-Human CD4 Purified (Product # 14-0049-82) (mouse IgG1 antibody) (purple histogram) followed by Anti-Mouse IgG1 APC. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.F1000ResearchFlow cytometry dataset for cells collected from touched surfaces.2.Analytical and bioanalytical chemistryAnalysis of cellular autofluorescence in touch samples by flow cytometry: implications for front end separation of trace mixture evidence.3.Cell reportsComparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.4.BiomaterialsMesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing invivo. 5.Journal of immunology (Baltimore, Md. : 1950) ATAD5 deficiency decreases B cell division and Igh recombination.

技术参数

产品应用 Flow;IHC(F)

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