CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eFluor 450, eBioscience™

货号：48-9987-42,48-9987-41

规格：100 tests,25 tests

价格：3163,0

产品类型：流式抗体

品牌：eBioscience

抗原：CD235a

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HIR2 (GA-R2)

荧光染料：eFluor 450

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 450, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test（Flow）

Product Specific InformationDescription: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of diluted normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (48-9987-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (blue histogram) or Anti-Human CD235a (Glycophorin A) eFluor® 450 (purple histogram). All non-leukocytes were used for analysis.

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参考文献：

1.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma.2.Stem cell reportsGenetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.3.NaturePPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.4.Stem cells (Dayton, Ohio)EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.5.Nature medicineAltered translation of GATA1 in Diamond-Blackfan anemia.

技术参数

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