Ly-6A/E (Sca-1) Monoclonal Antibody (D7), eBioscience™

货号：14-5981-82,14-5981-85,14-5981-81

规格：100 µg,500 µg,50 µg

价格：1044,2564,0

产品类型：流式抗体

品牌：eBioscience

抗原：Sca-1

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：D7

荧光染料：Purified

类型:	单抗	同型对照：	IgG2a, kappa
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated D7 is recommended for use in flow cytometry.).Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (14-5981-82) in FlowStaining of 3-day Con A-stimulated C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse Ly-6A/E (Sca-1) Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Total viable cells were used for analysis.

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参考文献：

1.eLifeDifferential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.2.Nature communicationsStem cell factor is selectively secreted by arterial endothelial cells in bone marrow.3.Nature medicineBone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease.4.PloS oneZoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.5.The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

技术参数

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