CD95 (APO-1/Fas) Monoclonal Antibody (EOS9.1), Functional Grade, eBioscience™/CD95 (APO-1/Fas) 单克隆 (EOS9.1), 功能抗体, eBioscience™

货号：16-0958-81,16-0958-85

规格：50 µg,500 µg

价格：1196,4334

产品类型：流式抗体

品牌：eBioscience

抗原：CD95

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：EOS9.1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgM, kappa
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The EOS9.1 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. EOS9.1 does not block binding of DX2, another antibody specific for human CD95.Applications Reported: The EOS9.1 antibody has been reported for use in flow cytometric analysis. EOS9.1 is also effective in inducing apoptosis in in vitro functional studies.Applications Tested: The EOS9.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.仅用于科研。不用于诊断过程。

数据

CD95 (APO-1/Fas) Antibody (16-0958-81) in FlowJurkat cells were left unstimulated (left) or stimulated for 20 hours with Anti-Human CD95 (APO-1/Fas) Functional Grade Purified coated at 5 µg/mL in a 24-well culture plate (right).The stimulated cells were then harvested and stained sequentially with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and the Annexin V Apoptosis Detection Kit eFluor® 450 (Product # 88-8006-72), then fixed and permeabilized with the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained with Anti-Human PARP1 (Cleaved) PE (Product # 12-6668-42).Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.

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参考文献：

IF1.Proceedings of the National Academy of Sciences of the United States of AmericaGenetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis."Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 16-0958-81) in Immunofluorescence"流式2.Leukemia researchEstrogen treatment induces MLL aberrations in human lymphoblastoid cells."16-0958 was used in Flow cytometry/Cell sorting to conclude that concentrations of E2 and 4-OH-E2 that may occur during pregnancy, or during use of oral contraceptives, can cause aberrations of the MLL gene."AuthorsSchnyder S,Du NT,Le HB,Singh S,Loredo GA,Vaughan ATFN3.Hepatology (Baltimore, Md.)Hepatitis C virus acts as a tumor accelerator by blocking apoptosis in a mouse model of hepatocarcinogenesis."16-0958 was used in Functional assays to evaluate the effect of viral proteins on apoptosis in HepG2 cells in which apoptosis was induced by anti-Fas antibody."AuthorsKamegaya Y,Hiasa Y,Zukerberg L,Fowler N,Blackard JT,Lin W,Choe WH,Schmidt EV,Chung RT

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