Alpha-Smooth Muscle Actin Polyclonal Antibody

货号：PA5-16697

规格：500 µL

价格：4885

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：		用法：	2 µg/mL（ICC）；2 µg/mL（IF）；2 µg/mL（IHC（P））；1-3 µg/mL（WB）
免疫原性：	A synthetic peptide from N-terminus of human alpha-smooth muscle isoform of actin

产品详细信息PA5-16697 targets Actin Smooth Muscle in IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples.The PA5-16697 immunogen is a synthetic peptide from N-terminus of human alpha-smooth muscle isoform of actin.靶标信息Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

数据

Alpha-Smooth Muscle Actin Antibody (PA5-16697) in IHCFormalin-fixed, paraffin-embedded human leiomyoma stained with Actin using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells. Alpha-Smooth Muscle Actin Antibody (PA5-16697) in IFImmunofluorescent analysis of Actin Smooth Muscle was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Actin Smooth Muscle Rabbit Polyclonal Antibody (Product # PA5-16697) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeleton localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Alpha-Smooth Muscle Actin Antibody (PA5-16697) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), K-562 (Lane 2) and MDA-MB-231 (Lane 3). The blots were probed with Anti-Actin smooth muscle Rabbit Polyclonal Antibody (Product # PA5-16697, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 49 kDa band corresponding to Actin smooth muscle was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Human liver regeneration in advanced cirrhosis is organized by the portal tree."PA516697 was used in immunohistochemistry - paraffin section to examine the vascular alterations in the areas of parenchymal extinction"2.Neutralizing murine TGFβR2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis."PA5-16697 was used in immunohistochemistry - paraffin section to analyze promotion of a differentiated tumor cell phenotype and inhibition of pancreatic cancer metastasis by neutralizing murine TGF-betaR2"3.Smooth Muscle-Targeted Overexpression of Peroxisome Proliferator Activated Receptor-γ Disrupts Vascular Wall Structure and Function."PA5-16697 was used in western blot to learn about disruption of vascular wall structure and function by smooth muscle-targeted overexpression of peroxisome proliferator activated receptor-gamma"4.Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis."PA5-16697 was used in immunohistochemistry - frozen section to study platelet CLEC-2 and vascular smooth muscle cells that stimulate platelets and facilitate thrombus formation and implications in atherothrombosis"5.PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours."PA5-16697 was used in western blot to elucidate the potential of PIK3CA to induce multipotency during tumorigenesis in the mammary gland."

技术参数

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