Pierce TCEP-HCl, No-Weigh Format TCEP-HCl还原剂，无需称量
货号：A35349
规格：10 x 1 mg
价格：1600
产品类型：蛋白凝胶电泳
品牌：Thermo Fisher
| Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability. |
| 特点： |
| - Odorless—Unlike DTT or BME, TCEP is odor-free, so it can reduce proteins conveniently on the bench; contributes to a healthier lab environment- Specific—selective and complete reduction of even the most stable water-soluble disulfides over a wide pH range- Simple—effective reduction at room temperature and pH 5 in less than five minutes- Stable—it's inherent stability and resistant to air oxidation eliminates the need for any special precautions while handling or storing; non-volatile and non-reactive toward other functional groups found in proteins- Efficient—For most applications, 5 to 50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.- Compatible—With TCEP, removal of the reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations). |
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