Nkx2.5 Polyclonal Antibody

货号： PA5-19138

规格：100 µg

价格：4443

产品类型：免疫组化一抗

品牌：iBiochips

物种：人/小鼠/大鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay Dependent(IF)；3.75 µg/mL（IHC）；1:32000（pep-ELISA）；0.3-1 µg/mL(WB)
免疫原性：	Synthetic peptide sequence (PRAYSDPDPAKDPR) corresponding to the internal amino acids of NKX2-5

产品详细信息This antibody is predicted to react with bovine and canine based on sequence homology.靶标信息This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.

数据

Nkx2.5 Antibody (PA5-19138) in WBWestern Blot staining of Human Heart lysate using Product # PA5-19138 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence. Nkx2.5 Antibody (PA5-19138) in IFImmunofluorescence analysis of Nkx2.5 in U2OS cells using a Nkx2.5 monoclonal antibody (Product # PA5-19138) at 5 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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