Green Fluorescent Dye/绿色荧光染料
货号：30078-1,30078-2
规格：500 Reactions,200 Reactions
价格：866,337
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| LavaLAMP™ DNA amplification products may be detected by agarose gel electrophoresis or by realtime/end-point monitoring with fluorescent double-stranded DNA-binding dyes, such as the Lucigen Green Fluorescent Dye (Cat. No. 30078-1). Turbidity may also be monitored to assess amplification (Mori, 2001), but this method is less sensitive. LavaLAMP™DNA扩增产物可通过琼脂糖凝胶电泳或荧光双链DNA结合染料（如Lucigen Green荧光染料（货号30078-1））进行实时/终点监测来检测。 也可以监测浊度以评估扩增（Mori，2001），但是这种方法不那么敏感。 |
| 数据： |
| Figure 1. Effect of primer set and temperature on LAMP results. Three primer sets were screened across a temperature range of 68°C – 74°C with recommended 1X default reaction conditions. Real-time detection was performed on a CFX96 Thermal Cycler (Bio-Rad) using Green Fluorescent Dye (Lucigen). Primer Set 3 provided the fastest positive Time to Result with the least amount of background amplification. Within Primer Set 3, 74°C provided the best resolution between the positive and negative samples. Note: Exact temperatures were set by the CFX96 Thermal Cycler instrument within the selected temperature range. Specific temperatures tested will depend on the real-time instrument used.图1.引物组和温度对LAMP结果的影响。 在推荐的1X默认反应条件下，在68°C – 74°C的温度范围内筛选了三套引物。使用绿色荧光染料（Lucigen）在CFX96热循环仪（Bio-Rad）上进行实时检测。 引物组3提供了最快的正结果时间，而背景扩增量最少。 在引物组3中，74°C在正负样品之间提供了最佳分辨率。 注意：精确温度是由CFX96热循环仪在选定的温度范围内设置的。 测试的特定温度将取决于所使用的实时仪器。Fluorescent Signals from Different LAMP ReactionsFigure 2: Early Fluorescent Signals from Positive LAMP Reactions and Late Background Signals from NTC Controls after Extended Incubation. LAMP reactions were run in a real-time thermal cycler. The fluorescent signal from each reaction was captured over a 60-minute reaction time. The red and blue lines represent the fluorescent signals from Positive Control reactions with varying amounts of target. The black lines represent the non-specific background amplification signals that arise later in No Target Control reactions.图2：长时间孵育后，来自阳性LAMP反应的早期荧光信号和来自NTC对照的晚期背景信号。 LAMP反应在实时热循环仪中进行。 在60分钟的反应时间内捕获了每个反应的荧光信号。 红线和蓝线表示来自阳性对照反应的荧光信号，靶标量不同。 黑线表示非特异性背景扩增信号，该信号稍后在“无靶标对照”反应中出现。 |
| 相关产品： |
| ▪LavaLAMP™ DNA Component Kit with Dye（货号#30067-1）▪ LavaLAMP™ DNA Component Kit（货号#30076-1） |
| 参考文献： |
| 1.Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000 28(12):E63. 2.Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002 16(3):223-9. |
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