NxGen® T4 DNA Ligase（Low Concentration)/NxGen® T4 DNA连接酶（低浓度）-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

NxGen® T4 DNA Ligase（Low Concentration)/NxGen® T4 DNA连接酶（低浓度）

2024-10-17

NxGen® T4 DNA Ligase（Low Concentration)/NxGen® T4 DNA连接酶（低浓度）

货号：30241-1

规格：1500 U

价格：1870

产品类型：Modifying Enzymes连接酶

品牌：Lucigen

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.NxGen T4 DNA 连接酶是一种常用于DNA克隆的ATP依赖性连接酶。它可以共价连接由限制酶消化或其他酶促过程产生的具有5^，-磷酸化和3^，-羟基化的平末端或相容性粘性末端的dsDNA分子。它对单链核酸没有活性。

优点：

▪Reliably join double-stranded nucleic acid chains.

数据：

Figure 1. Purity.The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining. Molecular weight markers are in Lane 1. T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).图 1.纯度。通过SDS-聚丙烯酰胺凝胶电泳和考马斯亮蓝染色判断，T4 DNA连接酶的纯度等于或大于95％。分子量标记在泳道1中。T4 DNA连接酶的上样量为5个单位（通道2）和10个单位（通道3）。

Figure 2. Exonuclease and Endonuclease Activity Assay. The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6). Molecular weight markers are in Lanes 1 and 2.图 2.核酸外切酶和核酸内切酶活性测定。 T4 DNA连接酶不含可检测的核酸外切酶和核酸内切酶活性，这是通过将琼脂糖凝胶电泳在37°C下将10个单位的酶与1 µg HindIII消化的λDNA一起温育16小时后确定的（第3条和第4条）和超螺旋的pUC19 DNA（泳道5和6）。分子量标记在泳道1和2中。

组成成分：

▪Low Concentration Ligase▪10X T4 DNA Ligase Buffer

NxGen® T4 DNA Ligase（Low Concentration)/NxGen® T4 DNA连接酶（低浓度）

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