NxGen® T4 DNA Ligase High Concentration Rapid Kit NxGen®T4DNA连接酶快速高浓度试剂盒
货号：30243-1
规格：1500 U
价格：2865
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. NxGEN T4 DNA连接酶是一种常用于DNA克隆的ATP依赖性连接酶。它可以共价连接由限制酶消化或其他酶促过程产生的具有5‘-磷酸化和3'-羟基化的平末端或相容性粘性末端的dsDNA分子。它对单链核酸没有活性。 |
| 优点： |
| ▪ Reliably join double-stranded nucleic acid chains. |
| 数据： |
| Figure 1: Purity.The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining. Molecular weight markers are in Lane 1. T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).Figure 2:Exonuclease and Endonuclease Activity Assay. The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6). Molecular weight markers are in Lanes 1 and 2. |
| 组成成分： |
| ▪HighConcentration Ligase▪10X T4 DNA Ligase Buffer▪ 2X Rapid Ligation Buffer |
| 相关产品： |
| ▪NxGen® T4 DNA Ligase （LowConcentration）货号#30241-1） |
技术参数 产品优点 - Reliably join double-stranded nucleic acid chains.
产品应用 - The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
- 连接平末端或粘性末端的DNA片段
- 修复双链核酸中的切口
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