PDGFRA Recombinant Polyclonal Antibody

货号： 710169

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：eBioscience

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-3 µg/mL (ICC)；1-3 µg/mL (IF)；1 µL（ChIP）；2-5 µg/mL (WB)
免疫原性：	Recombinant protein corresponding to amino acids 259–436 of human platelet-derived growth factor receptor – alpha chain

产品详细信息Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. 靶标信息PDGFR-alpha belongs to the protein tyrosine kinase family, and binds to the alpha chain of PDGF. Members of the PDGF receptor subfamily are cell surface receptors that act as mitogens for cells of mesenchymal origin, and consist of alpha and beta isoforms of ~190, and 125 kDa, respectively. Activated PDGF receptors act on signaling pathways that control cell growth, actin reorganization, migration, and differentiation. In humans, the gene is located on the q arm of chromosome 4.

数据

PDGFRA Antibody (710169) in IF Immunofluorescent analysis of PDGFR alpha in serum-starved HeLa cells treated with insulin (100 ng/mL, 15 min) using a PDGFR alpha Recombinant Rabbit Polyclonal Antibody (Product # 710169) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha. PDGFRA Antibody (710169) in WB Western blot analysis of PDGFR alpha in whole cell extracts of HepG2 cells using a PDGFR alpha Recombinant Rabbit Polyclonal Antibody (Product # 710169) at a dilution of 2.5 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~190kDa. PDGFRA Antibody (710169) in ChIP Chromatin immunoprecipitation analysis of PDGF Receptor alpha was performed using cross-linked chromatin from 1 x 10^6 HCT116 human colon carcinoma cells treated with serum for 0, 15, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a PDGF Receptor alpha Recombinant Rabbit Polyclonal Antibody (Product # 710169). Chromatin aliquots from ~1 x 10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -3.2kb upstream of the human FOS gene, or exon-4 of human FOS. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the FOS locus is shown above the data where boxes represent exons (grey boxes = translated regions, white boxes = untranslated regions), the zigzag lines represent introns, and the straight line represents upstream sequence. Regions amplified by FOS primers are represented by black bars. Data courtesy of the Innovators Program.

二抗荧光二抗免疫组化一抗标签抗体

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技术参数

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