Terminator™ 5'-Phosphate-Dependent Exonuclease/Terminator™5'-磷酸依赖性外切核酸酶
货号: TER51020
规格:40 U @ 1 U/ul
价格:3365
产品类型:核酸酶和抑制剂
品牌:Lucigen
Terminator™ 5′-Phosphate-Dependent Exonuclease is a processive 5′→3′ exonuclease that digests RNA having a 5′ monophosphate. It does not digest RNA having a 5′-triphosphate, 5′-cap, or 5′-hydroxyl group. These enzymatic properties make Terminator Exonuclease ideal for producing mRNA-enriched samples from both eukaryotic and prokaryotic total RNA preparations by selectively digesting the ribosomal RNA (rRNA). Terminator™5'-磷酸盐依赖性核酸外切酶是一种消化性5'→3'核酸外切酶,可消化具有5'一磷酸的RNA。 它不消化具有5'-三磷酸,5'-cap或5'-羟基的RNA。 这些酶学特性使终止子核酸外切酶非常适合通过选择性消化核糖体RNA(rRNA)从真核和原核总RNA制备物中制备富含mRNA的样品。Terminator Exonuclease can be used to isolate eukaryotic mRNA substantially free of 18S and 28S rRNA without using an oligo(dT) matrix. Further, Terminator Exonuclease provides a simple and effective method for purifying unprocessed, primary bacterial transcripts by removing large rRNA and other processed transcripts. 终止子核酸外切酶可用于分离基本不含18S和28S rRNA的真核mRNA,而无需使用oligo(dT)基质。 此外,终止子核酸外切酶提供了一种简单有效的方法,可通过去除大的rRNA和其他加工的转录本来纯化未加工的原始细菌转录本。Terminator Exonuclease will also digest single-stranded DNA (ssDNA) having a 5′-phosphate group. It does not digest ssDNA or dsDNA having a 5′-triphosphate or a 5′-hydroxyl group. Terminator Exonuclease is not inhibited by RNase Inhibitors such as RNasin®, Prime RNase Inhibitor™, or Epicentre’s RiboGuard™ RNase Inhibitor. 终止子核酸外切酶还将消化具有5'-磷酸基团的单链DNA(ssDNA)。 它不消化具有5'-三磷酸或5'-羟基的ssDNA或dsDNA。 终结者核酸外切酶不受RNase抑制剂(如RNasin®,Prime RNase Inhibitor™或Epicentre的RiboGuard™RNase抑制剂)的抑制。Terminator 5′-Phosphate-Dependent Exonuclease is available in a 40-U size at a concentration of 1 U/µL. Two different Terminator 10X Reaction Buffers are also provided with the enzyme, each optimized for different applications. 终止剂5'-磷酸盐依赖性核酸外切酶的40-U大小的浓度为1 U / µL。 酶还提供了两种不同的Terminator 10X反应缓冲液,每种都针对不同的应用进行了优化。Terminator Exonuclease是一种5’→3’进行性核酸外切酶,可降解带有5’单磷酸的RNA。它不会降解具有5’三磷酸、5’-帽结构(例如大多数真核mRNA带有的5’-帽结构)或5’OH的RNA。它也消化带有5’磷酸的单链DNA,不消化具有5'-三磷酸或5'-羟基的ssDNA或dsDNA。该酶不受蛋白形式的RNase抑制剂的抑制。 |
组成成分: |
▪ Terminator™ Exonuclease:储存在-20°C▪ Terminator™ 10X Reaction Buffer A:储存在-20°C▪ Terminator™ 10X Reaction Buffer B:储存在-20°C |
相关产品: |
▪ Riboguard™ RNase Inhibitor(#RG90910K/ RG90925)▪ Sterile Nuclease-Free Water(#W7350ML) |
技术参数 产品优点 - Specific: Processively digests RNA with 5´-monophosphate ends but not RNAs with 5´-triphosphate, 5´-cap or 5´-hydroxyl groups starting from the 5´ end - Flexible: Use this enzyme to help characterize 5´-ends of RNA and to enrich for mRNA in prokaryotic or eukaryotic total RNA preparations.- 特定:从5´末端开始,消化具有5´-单磷酸末端的RNA,但不消化具有5´-三磷酸,5´-帽或5´-羟基的RNA。- 灵活:使用此酶可帮助鉴定RNA的5'末端并丰富原核或真核总RNA制备物中的mRNA。
产品应用 - 鉴定RNA转录本的5’末端 - 在1小时内从真核或原核总RNA制备物中制备富集了mRNA的样品,无需使用oligo(dT)、树脂或磁珠
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