| RNase I preferentially degrades single-stranded RNA to individual nucleoside 3′ monophosphates by cleaving every phosphodiester bond.By comparison, other ribonucleases cleave only after specific residues (e.g., RNase A cleaves 3′ to pyrimidine residues).Thus, RNase I is useful for removing RNA from DNA preparations,detecting mismatches in RNA:RNA and RNA:DNA hybrids, and analyzing and quantifying RNA in ribonuclease protection assays (RPA).The enzyme is completely inactivated by heating at 70°C for 20 minutes in the presence of 5 mM dithiothreitol (DTT), eliminating the requirement to remove the enzyme prior to many subsequent procedures. 通过在所有二核苷酸对之间切割，RNase I通过2’,3’环状单磷酸酯中间体将ssRNA降解为核苷-3’-单磷酸。通过在70℃条件下加热20分钟可以使该酶完全失活，因而在进行许多下游应用之前无需进行除去酶的步骤。 |
| 1.Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501. 2.Winter, E. et al., (1985) Proc. Natl. Acad. Sci. USA 82, 7575. 3.Myers, R.M. et al., (1985) Science 230, 1242.4.Sambrook, J. et al., (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. 5.Saccomanno, C.F. et al., (1992) BioTechniques 13, 847. |
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