IL-21 Monoclonal Antibody (FFA21), Functional Grade

货号：16-7211-82,16-7211-85

规格：100μg,500μg

价格：1752,4228

产品类型：抗体和染料

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：FFA21

荧光染料：其它

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade
浓度：	1mg/mL	用法：	2.0 µg/mL(ICC);10 µg/mL(IF);1 µg/mL(WB)

产品详细信息Description: This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells.In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (cat. 51-7213).Applications Reported: The monoclonal antibody FFA21 reacts with and inhibits the bioactivity of mouse IL-21.Applications Tested: The ND50 of FFA21, as determined by the inhibition of recombinant mouse IL-21-induced proliferation of the B9 cell line, is 0.2-0.5 µg/mL in the presence of 0.5 ng/mL recombinant mouse IL-21. The neutralization dose of this antibody will vary depending on cell type, assay method, and concentration of cytokine.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

数据

IL-21 Antibody (16-7211-82) in FNNeutralization of Mouse IL-21 Recombinant Protein activity as measured by B9 cell proliferation.

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参考文献：

1.Frontiers in immunologyAge-Related Differential Stimulation of Immune Response byBabesia microtiandBorrelia burgdorferiDuring Acute Phase of Infection Affects Disease Severity."Published figure using IL-21 monoclonal antibody (Product # 16-7211-82) in Flow Cytometry"2.The Journal of clinical investigationCirculating T follicular regulatory and helper cells have memory-like properties."16-7211 was used in Flow cytometry/Cell sorting to indicate circulating memory-like Tfr cells are less suppressive than LN Tfr cells and circulating memory-like Tfh cells are more potent than LN effector Tfh cells."AuthorsSage PT,Alvarez D,Godec J,von Andrian UH,Sharpe AH3.Nature communicationsHepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance."Published figure using IL-21 monoclonal antibody (Product # 16-7211-82) in ELISA"4.Nature communicationsHepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance."Published figure using IL-21 monoclonal antibody (Product # 16-7211-82) in ELISA"5.Proceedings of the National Academy of Sciences of the United States of AmericaInterleukin-6/interleukin-21 signaling axis is critical in the pathogenesis of pulmonary arterial hypertension."16-7211 was used in Neutralization experiments to suggest that IL-21 promotes PAH in association with M2 macrophage polarisation, downstream of IL-6-signalling."AuthorsHashimoto-Kataoka T,Hosen N,Sonobe T,Arita Y,Yasui T,Masaki T,Minami M,Inagaki T,Miyagawa S,Sawa Y,Murakami M,Kumanogoh A,Yamauchi-Takihara K,Okumura M,Kishimoto T,Komuro I,Shirai M,Sakata Y,Nakaoka Y

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