MasterPure™ Yeast DNA Purification Kit MasterPure™酵母DNA纯化试剂盒

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MasterPure™ Yeast DNA Purification Kit MasterPure™酵母DNA纯化试剂盒

货号:MPY80200

规格:200 Purifications

价格:5085

产品类型:DNA提取

品牌:Lucigen

The MasterPure™ Yeast DNA Purification Kit provides all of the reagents necessary to consistently isolate highly purified DNA from a variety of yeast species including: Candida, Saccharomyces, Pichia, and Schizosaccharomyces. In addition, users can isolate DNA from filamentous fungal species such as Aspergillus1 and Penicillium. This kit utilizes a nonenzymatic approach to lyse the yeast cell wall combined with a rapid precipitation step to remove contaminating macromolecules. The purified DNA can then be used in many applications including hybridization, restriction enzyme digestion, PCR amplification, and next-generation sequencing. We offer several products for PCR that incorporate the MasterAmp™ PCR Enhancement Technology+, which substantially improves product yield and decreases nonspecific product formation.
优点:
▪ Fast: Obtain purified DNA in less than 40 minutes▪ Flexible: Purify DNA from a wide variety of yeast species, including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus2 and Penicillium▪ High Quality DNA: Purify high molecular weight genomic DNA that is ready to use in many molecular biology applications, including PCR amplification, restriction endonuclease digestion, Southern blotting, genomic library preparation and fungal identification and typing▪ High Yields: Purify more yeast genomic DNA than other commercially available kits▪ Simple: No enzymatic lysis, bead beating, columns, phenol or other organic solvents are used.
组成成分:
▪ TE Buffer:储存在室温▪ RNase A (5 μg/μL):储存在室温▪ MPC Protein Precipitation Reagent:储存在室温▪ Yeast Cell Lysis Solution:储存在室温
参考文献:
1. Jin, J. et al., (2004) J. Clin. Microbiol. 42, 4293. 2. Ausubel, F. et al., (eds.) (1995) Current Protocols in Molecular Biology (CD ROM ver. 3.7.5) John Wiley and Sons, New York, Appendix 3D. 3. Sherman, F. (1991) Meth. Enzymol. 194, 3.

技术参数

产品优点 - Fast: Obtain purified DNA in less than 40 minutes - Flexible: Purify DNA from a wide variety of yeast species, including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus2 and Penicillium - High Quality DNA: Purify high molecular weight genomic DNA that is ready to use in many molecular biology applications, including PCR amplification, restriction endonuclease digestion, Southern blotting, genomic library preparation and fungal identification and typing - High Yields: Purify more yeast genomic DNA than other commercially available kits Simple: No enzymatic lysis, bead beating, columns, phenol or other organic solvents are used

产品应用 - The purified DNA can then be used in many applications including hybridization, restriction enzyme digestion, PCR amplification, and next-generation sequencing.

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