OTX2 Recombinant Polyclonal Antibody (14HCLC)

货号： 710981

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：eBioscience

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/mL (ICC)；1 µg/mL (IF)；0.5-1µg/mL (WB)
免疫原性：	Multiple peptides corresponding to Human OTX2 (aa 20-39; 276-289)

产品详细信息This antibody is predicted to react with Rat, Mouse, Rabbit and Pig.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. 靶标信息OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

数据

OTX2 Antibody (710981) in IF Immunofluorescence was performed on fixed and permeabilized NTERA-2 cells for detection of OTX2 using Anti-OTX2 Recombinant Rabbit Polyclonal Antibody (Product # 710981, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of OTX2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of OTX2. Panel e) represents control cells with no primary Antibody to assess background. OTX2 Antibody (710981) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of NTERA-2 (Lane1) and nuclear enriched extract of NTERA-2 (Lane2). The blots were probed with Anti-OTX2 Recombinant Rabbit Polyclonal Antibody (Product # 710981, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 35 kDa band corresponding to OTX2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

二抗荧光二抗免疫组化一抗标签抗体

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