Expresso® Solubility and Expression Screening System + Accura® High-Fidelity Polymerase，Expresso®溶解度和表达筛选系统+Accura®高保真聚合酶
货号：49064-1
规格：24 rxns
价格：13098
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The Expresso® Solubility and Expression Screening System is an all-in-one system that enables PCR amplification, rapid cloning, and expression of your protein of interest with a diverse panel of powerful and cleavable solubility tags. Lucigen has identified 7 unique and literature-validated solubility tags and combined them with Expr essioneering™ technology to create a suite of cloning-ready vectors for rapid and parallel evaluation of your protein with all 7 solubility tags (plus a 6xHis-tagged control vector). |
| 优点： |
| ▪ Use one amplicon to simultaneously generate all your constructs；▪Rapid, simultaneous protein screening with TEV-cleavable fusion tags；▪ PCR to protein in as little as 4 days； |
| 数据： |
| Figure 1.Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added. Fusion tags are cleavable via a TEV protease site.Figure 2.GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion. Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet |
| 相关产品： |
| ▪Expresso® Solubility and Express. Screening System（货号#49060-1） |
技术参数 产品优点 - Use one amplicon to simultaneously generate all your constructs - Rapid, simultaneous protein screening with TEV-cleavable fusion tags - PCR to protein in as little as 4 days
产品应用 - One convenient screening kit containing 7 fusion tags proven to increase protein expression and solubility
组成成分 The Expresso Solubility and Expression Screening System contains pre-processed pSol™ vectors with 7 different fusion tags and one HIS-tagged control vector (3 reactions of each vector), positive control insert, sequencing primers, E. cloni® 10G Chemically Competent Cells (SOLOs), and 20% Rhamnose solution and 15% Glucose solution autoinduction reagents. SelecTEV™ Protease, 20% Rhamnose Solution and 15% Glucose Solution are available for purchase separately.
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