CD134 (OX40) Monoclonal Antibody (OX-86), Functional Grade, eBioscience™/CD134 (OX40) 单克隆 (OX-86), 功能抗体, eBioscience™

货号：16-1341-85,16-1341-025,16-1341-050

规格：500 µg,25 mg,50 mg

价格：3735,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD134

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：OX-86

荧光染料：Alexa Fluor 488

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses,regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis. It does not block binding of CD134 to its ligand, but has been reported for use in functional assays.Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.仅用于科研。不用于诊断过程。

数据

CD134 (OX40) Antibody (16-1341-85) in FlowStaining of Con A stimulated mouse splenocytes with Anti-Mouse CD134 (OX40) PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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参考文献：

流式1.Journal of immunology (Baltimore, Md. : 1950)The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice."Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry"2.Nature immunologyLate stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling."16-1341 was used in Flow cytometry/Cell sorting to discover that both NF-κB and tonic interferon signals are involved in the final maturation of thymocytes into naive T cells."AuthorsXing Y,Wang X,Jameson SC,Hogquist KA3.Nature immunologyCostimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells."16-1341 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells.."AuthorsMahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA功能检测4.Journal of immunology (Baltimore, Md. : 1950)The number of responding CD4 T cells and the dose of antigen conjointly determine the TH1/TH2 phenotype by modulating B7/CD28 interactions."16-1341 was used in Functional assays to show that a greater number of Ag-dependent CD4 T cell interactions are required to generate Th2 than Th1 cells."AuthorsRudulier CD,McKinstry KK,Al-Yassin GA,Kroeger DR,Bretscher PA5.European journal of immunologyTNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40."16-1341 was used in Functional assays to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40."AuthorsHamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen X中和6.Mucosal immunologyRetinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells."16-1341 was used in Neutralization experiments to investigate the role of retinoic acid in mesenteric lymph node dendritic cells."AuthorsYokota-Nakatsuma A,Takeuchi H,Ohoka Y,Kato C,Song SY,Hoshino T,Yagita H,Ohteki T,Iwata M

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