PAX3 Polyclonal Antibody

货号：PA1-107

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：	1 mg/m	用法：	1：100-1：1000 (ICC)；1：100-1：1000 (IF)；1:100-1:1000（IHC (F)）；1:500-1:1000 (WB) ；2µg（IP）
免疫原性：	Amino acids 301-442 of human PAX3

产品详细信息PA1-107 has successfully been used in Western blot for detecting overexpressed and recombinant human Pax3 protein, but may not be suitable for detecting endogenous Pax3.By immunofluorescence, PA1-107 has been used to stain frozen embryonic mouse brain tissue.PA1-107 has been validated for IP using overexpressed Pax3 protein.靶标信息Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

数据

PAX3 Antibody (PA1-107) in IF Immunofluorescence was performed on mouse E10.5 embryo trunk at the forelimb level. Embryos were fixed with 4%PFA in 0.1M phosphate buffer, pH 7.2 at 4C for 30 minutes, processed for cryoprotection in 20% sucrose, and embedded in OCT compound for cryosectioning at 10 µm thickness. Following sectioning, tissues were washed in PBS with 0.1% Triton-X 100 (PBT), blocked in 1% goat serum in PBT for 30 minutes at room temperature, and then probed with a Pax3 polyclonal antibody (Product # PA1-107) at a concentration of 1 µg/mL in blocking buffer overnight at 4C. Tissues were washed extensively with PBT, and detection was performed using an Alexa Fluor 568-conjugated goat anti-rabbit IgG secondary antibody (red) at a dilution of 1:1000 in blocking buffer. Nuclei (blue) were stained with DAPI. Note: Staining was observed in the dorsal neural tube (symmetric two halves on the left side of the image), the dermomyotome/myotome (middle streak between symmetric halves), and limb muscle progenitors (scattered cells to the right side of the image). Data supplied courtesy of the Innovator's Program. PAX3 Antibody (PA1-107) in IF Immunofluorescent analysis of Pax3 (red) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with blocking buffer included the kit for one hour at room temperature. Cells were stained with a Pax3 polyclonal antibody (Product # PA1-107) at a dilution of 1:200 in blocking buffer for 3 hours at room temperature, and then incubated with an AlexaFluor 594- conjugated donkey anti-rabbit IgG secondary antibody (Product # A-21207) at a dilution of 1:250 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification. PAX3 Antibody (PA1-107) in IP Immunoprecipitation of Pax3 was performed on RIPA cell extracts originating from a 6cm dish of HeLa cells transfected for 24 hours with a Pax3-HA construct. Cells were lysed in 1 mL of RIPA buffer, and antigen-antibody complexes were formed by incubating 225 µL of HeLa Pax-HA cell extract with 2 µg of a Pax3 polyclonal antibody (Product # PA1-107) and Protein A sepharose for 4 hours at 4C. The samples were washed extensively with RIPA buffer, and eluted by boiling in SDS sample buffer. Samples were resolved on an 8% Tris-Glycine gel alongside whole cell lysate (WCL) from empty vector and Pax3-HA-transfected HeLa cells, transferred to a nitrocellulose membrane, and blocked with 2% BSA in PBST for 10 minutes at room temperature. The membrane was probed with an anti-HA polyclonal antibody overnight at 4C, washed in TBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody. Chemiluminescent detection was performed using ECL substrate. Data supplied courtesy of the Innovator's Program.

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Neural development Netrin1/DCC signaling promotes neuronal migration in the dorsal spinal cord.2.eLife NOVA regulates Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord.3.Neural development Netrin1/DCC signaling promotes neuronal migration in the dorsal spinal cord.4.eLife NOVA regulates Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord.

技术参数

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