E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg / E. cloni 10G ELITE电感受态细胞> 2 × 10^10 cfu/µg
货号:60051-1,60051-2 ,60052-1 ,60052-2,60052-3,60052-4
规格:12 rxns (SOLOs),24 rxns (SOLOs),12 rxns (DUOs) ,24 rxns (DUOs),24 rxns (SixPacks),48 rxns (SixPacks)
价格:2342,4057,1790,3531,2705,4508
产品类型:感受态细胞
品牌:Lucigen
E. cloni 10G and 10GF' Electrocompetent Cells are E. coli strains optimized for high efficiency
transformation. They are ideal for cloning and propagation of BAC, cosmid, or plasmid clones. They
give high yield and high quality plasmid DNA due to the endA1 mutation. E. cloni 10G and10GF' contain the inactive mcr and mrr mutations, allowing methylated genomic DNA
that has been isolated directly from mammalian or plant cells to be cloned without deletions or
rearrangements. The rpsL mutation confers resistance to streptomycin. |
优点: |
▪Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.);▪Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable;▪Available in a range of high transformation efficiencies (5 × 109to 4 × 1010cfu/µg); |
数据: |
Figure 1.E. cloni10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).Figure 2.Transformation efficiency comparison ofE. cloni10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells) |
相关产品: |
▪E. cloni 10GF' ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg(货号#60061)▪ E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 10^9 cfu/µg(货号#60117)▪E. cloni 10G SUPREME Electrocompetent Cells > 4 × 10^10 cfu/µg(货号#60081/60080) |
技术参数 产品优点 - Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.) - Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable. - Available in a range of high transformation efficiencies (5 × 109 to 4 × 1010 cfu/µg). - Convenient packaging options.
产品应用 - E. cloni 10G and 10GF' Electrocompetent Cells are E. coli strains optimized for high efficiencytransformation.
组成成分 Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), or SixPacks (6 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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