MessageBOOSTER™ cDNA Synthesis Kit for qPCR MessageBOOSTER™ qPCR cDNA合成试剂盒
货号：MB060124
规格：24 Rxns
价格：5848
产品类型：RNA-Seq建库
品牌：Lucigen
| Figure 1. MessageBOOSTER™ cDNA Synthesis Kit for qPCR Procedure.The MessageBOOSTER™ cDNA Synthesis Kit for qPCR reaction amplifies the poly(A) RNA (mRNA) in a total RNA sample isolated from 1-50 eukaryotic cells to enable more sensitive and more reproducible qPCR of even low-abundance transcripts. Note: MessageBOOSTER is not suited for bacterial/prokaryotic total RNA samples. |
| 优点： |
| ▪ Perform RNA amplification on purified total RNA followed by cDNA synthesis and detection ▪ Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.▪ Get more data out of precious samples – use less RNA for more RT-qPCR reactions▪ Readily and reproducibly detect even low-abundance transcripts in RNA from a single cell (CT values <35 cycles) ▪ Collect small RNA samples less often. ▪ Fast protocol amplifies and synthesizes cDNA in only 1 day. |
| 数据： |
| Figure 2. qPCR results from cDNA produced from a single NRK cell.cDNA was made from a single NRK cell using the MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit and qPCR of the PBGD target was performed using undiluted(red),1:10 diluted(green),1:100 diluted(blue),and 1:1,000 diluted(purple) aliquots.The low-abundance PBGD transcript was readily detected. |
| 组成成分： |
| ▪ T7-Oligo(dT) Primer A:储存在-20℃▪ RiboGuard RNase Inhibitor (40 U/µL) :储存在-20℃▪ RT PreMix :储存在-20℃▪ DNA Polymerase PreMix :储存在-20℃▪ DNA Polymerase :储存在-20℃▪ Finishing Solution :储存在-20℃▪ Random Primers :储存在-20℃▪ RNase H :储存在-20℃▪MMLV-RT :储存在-20℃▪ IVT PreMix A :储存在-20℃▪ T7 RNA Polymerase :储存在-20℃▪ 10X Transcription Reaction Buffer :储存在-20℃▪ RNase-Free DNase I (1 U/µL) :储存在-20℃▪ Forward Control PCR Primer (12.5 µM) :储存在-20℃▪ Reverse Control PCR Primer (12.5 µM) :储存在-20℃▪ DTT (100 mM) :储存在室温▪ Nuclease-Free Water, Sterile :储存在-20℃▪ Poly(I) :储存在-20℃ ▪ NRK Total RNA Control (50 ng/µL) :储存在-80℃ |
| 参考文献： |
| 1. Van Gelder, R. N. et al., (1990) Proc. Natl. Acad. Sci. USA 87 (5), 1663. |
技术参数 产品优点 - Perform RNA amplification on purified total RNA followed by cDNA synthesis and detection - Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample. - Get more data out of precious samples – use less RNA for more RT-qPCR reactions - Readily and reproducibly detect even low-abundance transcripts in RNA from a single cell (CT values <35 cycles) - Collect small RNA samples less often. - Fast protocol amplifies and synthesizes cDNA in only 1 day.
产品应用 - Amplifies the poly(A) RNA (mRNA) in a total RNA sample isolated from 1-50 eukaryotic cells
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