3'-DMT-dA(Bz) Synthesis Column; 1000 Å
货号：CG1-1000i-1,CG1-1000i-2,CG1-1000i-5
规格：1 µmol,200 nmol,50 nmol
价格：84.42,70.35,56.28
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This synthesis column is packed with 3'-DMT-dA(Bz)-Suc-CPG, an inverse linkage CPG used to create a 3'-3' linkage on the end of an oligonucleotide. An inverted 3'-3' linkage is desirable when needing to block polymerase extension through the 3'-terminus of a PCR probe, additionally oligonucleotides with inverted 3'-linkages are used for some sequencing techniques. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| Chemical Name: 3'-DMT-dA(Bz)-Suc-CPG; 1000 ÅAppearance: White PowderExtinction Coefficient at 260 nm: 15200 |
| 产品用途： |
| Protecting group deblock conditions: 3'DMT groups detritylate slowly. Increase detritylation time by 50%.Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 313.2 |
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