Fast-Link™ DNA Ligation Kit，Fast-Link™ DNA连接试剂盒
货号：LK0750H,LK6201H
规格：50 rxns,100 rxns
价格：2093,3421
产品类型：质粒
品牌：Lucigen
| The Fast-Link™ DNA Ligation Kits provide reagents optimized for rapid and efficient ligation of DNA. Ligation reactions require incubation for as little as 5 minutes at room temperature. Ligation reactions of PCR products containing nontemplate-dependent 3′ A-overhangs require incubation for just 1 hour at 16°C. Applications include cloning DNA into prokaryotic or eukaryotic plasmid vectors and bacteriophage lambda vectors, and sample preparation for next-generation DNA sequencing.Fast-Link DNA Ligation Kit 提供极其快速、高校的DNA连接。粘性末端连接可在室温下5分钟内完成。含有非模板依赖性3'A-突出端的PCR产物的连接反应需要在16℃下孵育仅1小时。与其他连接酶不同，在转化电感受态或化学感受态细胞之前，不必对Fast-Link 连接反应进行脱盐。 |
| 优点： |
| ▪ Clone with speed and efficiency ▪ Transform into E. coli without clean-up or desalting |
| 数据： |
| Figure 1. Time course for a cohesiveend ligation using the Fast-Link™ Kit. Lambda Hind III markers were ligated in a standard Fast-Link reaction using 2 U of Fast-Link DNA Ligase (lanes 3-7). Lane 1, 1-kb ladder; Lane 2, no enzyme; Lanes 3-7, 5 min, 15 min, 30 min, 1 hr, 2 hr, respectively.Figure 2. Time course for blunt-end ligation using the Fast-Link™ Kit. pUC19 digested with Pvu II was ligated in a standard Fast-Link reaction using 2 U of FastLink DNA Ligase (lanes 2-6). Lane 1, no enzyme; Lanes 2-6, 5 min, 15 min, 30 min, 45 min, 1 hr, respectively; Lane 7, 1-kb ladder. |
| 组成成分： |
| ▪ Fast-Link DNA Ligase (2 U/µL):储存在-20℃▪ Fast-Link™ 10X Ligation Buffer :储存在-20℃▪ ATP (10 mM) :储存在-20℃ |
| 参考文献： |
| 1. Weiss, B. et al., (1968) J. Biol. Chem. 243, 4543. 2. Ausubel, F. et al., (eds.) 1995 Current Protocols in Molecular Biology (CD ROM ver. 3.7.5) John Wiley and Sons, New York, Appendix 3D. 3. Levis, R. (1995) in PCR Primer, Dieffenbach, C. W. and Dveksler, G. S. (eds.), CSH Laboratory Press, New York, 539. 4. Sambrook, J. et al., (1989) Molecular Cloning: A Laboratory Manual (2nd ed.) New York: Cold Spring Harbor Laboratory Press. 5. Dugaiczyk, A. et al., (1975) J. Mol. Biol. 96, 171 |
技术参数 产品优点 - Clone with speed and efficiency
- Transform into E. coli without clean-up or desalting
产品应用 - Cloning DNA into prokaryotic or eukaryotic plasmid vectors and bacteriophage lambda vectors
- Sample preparation for next-generation DNA sequencing.
- 平末端和PCR产物TA克隆
- 将下一代测序接头连接到到平末端DNA
- 基因组和cDNA克隆及亚克隆
- BAC/fosmid文库构建
- Linker连接
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18329.htm
QQ:1749072012