F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488-FluoroNanogold/F(ab')2-山羊抗小鼠IgG(H + L)二抗,Alexa Fluor 488-F

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F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488-FluoroNanogold/F(ab')2-山羊抗小鼠IgG(H + L)二抗,Alexa Fluor 488-FluoroNanogold

货号:A-24920

规格:1 mL

价格:9313

产品类型:荧光二抗

品牌:Thermo Fisher

抗原:Gamma Immunoglobin

物种:小鼠

宿主:山羊

抗体亚型:IgG

荧光染料:Alexa Fluor 488

类型:二抗同型对照:
浓度: 0.08 mg/mL用法:Assay Dependent(IHC);0.2-10 µg/mL(IM);(IHC (P))
产品详细信息Alexa Fluor 488 FluoroNanogold Fab' fragment of goat anti-mouse IgG is covalently conjugated with uniform, 1.4 nm-diameter gold clusters and our exceptionally bright, photostable Alexa Fluor 488 dye (which is spectrally similar to fluorescein). FluoroNanogold conjugates can be used as probes in fluorescence microscopy and, when used with our LI Silver Enhancement Kit (Cat. no. L-24919), in immunoblotting, light microscopy or electron microscopy.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据

Mouse IgG (H+L) Secondary Antibody (A-24920) in IHCHuman prostate tissue with adenocarcinoma imaged using an Alexa Fluor 488 FluoroNanogold conjugate. Tissues were labeled with an anti-cytokeratin antibody, followed by staining with the Alexa Fluor 488 FluoroNanogold Fab' fragment of goat anti-mouse IgG antibody (Product # A-24920). The fluorescence image in the left panel was taken with filters appropriate for fluorescein. The right panel shows a bright-field image of the same system, processed with the LI Silver (LIS) Enhancement Kit (Product # L-24919) to visualize the gold distribution. Image courtesy of Nanoprobes, Inc.
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参考文献:
1.Oncotarget

Targeting the cancer-associated fibroblasts as a treatment in triple-negative breast cancer.

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