beta-3 Tubulin Monoclonal Antibody (2G10-TB3), eFluor 570, eBioscience™
货号：41-4510-80
规格：25 µg
价格：2657
产品类型：免疫组化一抗
品牌：eBioscience
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：IgG2a, kappa
荧光染料：Purified
类型: | 单抗 | 同型对照： | |
浓度： | 0.2 mg/mL | 用法： | 5 µg/mL (ICC)；5 µg/mL (IF)；Assay Dependent（IHC） |
免疫原性： | |
产品详细信息Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network. Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals. Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone. Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells. Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III.Applications Reported: This 2G10-TB3 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This 2G10-TB3 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
靶标信息The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
数据 |
| beta-3 Tubulin Antibody (41-4510-80) in ICC Immunocytochemistry of fixed, permeablized SK-N-SH cells stained with 5 µg/mL of Mouse IgG2a, K Isotype Control eFluor® 570 (Product # 41-4724) (left) or 5 µg/mL of Anti-Beta Tubulin Class III eFluor® 570 (right). Nuclei are stained with DAPI. |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
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QQ:1749072012