beta-3 Tubulin Monoclonal Antibody (2G10-TB3), eBioscience™
货号：14-4510-80 ,14-4510-82
规格：25 µg,100 µg
价格：1575,3536
产品类型：免疫组化一抗
品牌：eBioscience
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：IgG2a, kappa
荧光染料：Purified
类型: | 单抗 | 同型对照： | |
浓度： | 0.5 mg/mL | 用法： | 5 µg/mL (ICC)；5 µg/mL (IF)；Assay Dependent（IHC(P)）；Assay Dependent（IHC(F)）；Assay Dependent (WB) ；Assay Dependent （IP） |
免疫原性： | |
产品详细信息Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network. Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals. Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone. Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells. Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III.Applications Reported: This 2G10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This 2G10 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
数据 |
| beta-3 Tubulin Antibody (14-4510-82) in ICC Immunocytochemistry of formaldehyde-fixed, permeabilized SK-N-SH cells stained with 5 µg/mL of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (left) or 5 µg/mL of Anti-Beta Tubulin Class III Purified (right) followed by Anti-Mouse TRITC. Nuclei are stained with DAPI. |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Molecular autism Role of miR-146a in neural stem cell differentiation and neural lineage determination: relevance for neurodevelopmental disorders. |
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