OverExpress™ C43(DE3) Electrocompetent Cells OverExpress™ C43(DE3)电感受态细胞
货号：60345-1,60345-2
规格：12 rxns (SOLOs),24 rxns (SOLOs)
价格：3782,6649
产品类型：感受态细胞
品牌：Lucigen
| OverExpress C41(DE3) and C43(DE3) Electrocompetent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including bacteria, yeast, plant, viruses, and mammals. OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins (1-5). The strain C41(DE3) was derived from BL21(DE3). This strain has a mutation that reduces the level of T7 RNAP activity, thereby preventing cell death associated with overexpression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein. It carries at least one additional mutation that provides a greater level of tolerance to toxic proteins. As in standard BL21(DE 3) strains, OverExpress C41(DE3 and C43(DE3) are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA polymerase gene under the control of the lacUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7- driven expression vectors. OverExpress C41(DE3) and C43(DE3) are also deficient in the lon and ompT proteases. Please note that in OverExpress cell lines, expression of the target protein is expected to be slightly lower than with BL21(DE3). |
| 优点： |
| ▪ Express genes cloned into any T7 vector with these BL21(DE3) derivatives；▪ Effective in expressing toxic & membrane proteins；▪ Cited in over 350 research articles； |
| 数据： |
| Figure 1.Green Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.Figure 2.Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins. |
| 相关产品： |
| ▪OverExpress C41(DE3) Chemically Competent Cells（货号#60442）▪ OverExpress C41(DE3) pLysS Chemically Competent Cells（货号#60444）▪OverExpress C43(DE3) Chemically Competent Cells（货号#60446） |
技术参数 产品优点 - Express genes cloned into any T7 vector with these BL21(DE3) derivatives - Effective in expressing toxic & membrane proteins - Cited in over 350 research articles - Recommended for membrane proteins with T7 vector - Have issues with insoluble protein? Check out our solution to increase protein solubility - Interested in a competent E. coli strain for routine protein expression?
组成成分 Each OverExpress kit contains Electrocompetent or Chemically Competent Cells in SOLO packaging (1 transformation per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, pAVD10 Verification Plasmid, and complete protocols. ComboPacks contain 3 reactions each of chemically competent C41(DE3), C43(DE3), C41(DE)pLysS, and C43(DE3)pLysS.
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