CopyControl Fosmid LBR Production Kit（停产）
货号：CCFOS110
规格：1 Kit
价格：7961
产品类型：二代测序-建库
品牌：Lucigen
| The CopyControl Cloning System, based on technology developed by Dr. Waclaw Szybalski 1-3 at the University of Wisconsin-Madison, combines the clone stability afforded by single-copy cloning with the advantages of high yields of DNA obtained by “ondemand” induction of the clones to high-copy number. CopyControl Fosmid clones can be induced from single-copy to 10-20 copies per cell to improve DNA yields for sequencing, fingerprinting, subcloning, in vitro transcription, and other applications. |
| 优点： |
| ▪ Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts ▪ Stabilize clones with copy number control▪ Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit |
| 组成成分： |
| ▪ End-It Enzyme Mix：-20℃▪ End-It 10X Buffer：-20℃▪ dNTP Mix (2.5 mM each) ：-20℃▪ ATP (10 mM) ：-20℃▪ Fast-Link DNA Ligase (2 U/µL) ：-20℃▪ End-It Enzyme Mix Fast-Link 10X Ligation Buffer：-20℃▪ GELase Enzyme Preparation (1 U/µL) ：-20℃▪ GELase 50X Buffer ：-20℃▪ Fosmid Control DNA (100 ng/µL) ：-20℃▪ pCC2FOS Fosmid Vector (0.5 µg/µL) ：-20℃▪ CopyControl Fosmid Autoinduction Solution (500X)：-20℃▪ Phage T1 Resistant EPI300 T1R Glycerol Stock ：-20℃▪ MaxPlax Lambda Packaging Extract ：-70℃▪ LE392MP Control Plating Strain Glycerol Stock ：-70℃▪ Ligated Lambda Control DNA (0.02 µg/µL) ：-70℃ |
| 数据： |
Figure 1. Production of a CopyControl™ Fosmid Library and subsequent inductin of clones to high-copy number. |
技术参数 产品优点 - Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts
- Stabilize clones with copy number control
- Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit
产品应用 - CopyControl Fosmid clones can be induced from single-copy to 10-20 copies per cell to improve DNA yields for sequencing, fingerprinting, subcloning, in vitro transcription, and other applications.
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