CopyControl HTP Fosmid LBR Production Kit
货号：CCFOS059
规格：1 Kit
价格：10264
产品类型：二代测序-建库
品牌：Lucigen
| The CopyControl Cloning System, based on technology developed by Dr. Waclaw Szybalski 1-3 at the University of Wisconsin-Madison, combines the clone stability afforded by single-copy cloning with the advantages of high yields of DNA obtained by “ondemand”induction of the clones to high-copy number. CopyControl Fosmid clones can be induced from single-copy to 10-20 copies per cell to improve DNA yields for sequencing, fingerprinting, subcloning, in vitro transcription, and other applications. 基于威斯康星大学麦迪逊分校Waclaw Szybalski 博士开发的技术的CopyControl克隆系统将单拷贝克隆提供的克隆稳定性与“按需”诱导DNA的高产量优势相结合。 克隆到高拷贝数。 可以将CopyControl Fosmid克隆从每细胞单拷贝复制到10-20拷贝，以提高DNA产量，用于测序，指纹分析，亚克隆，体外转录和其他应用。 |
| 优点： |
| ▪ Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts ▪ Stabilize clones with copy number control▪Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit ▪ 使用完整的试剂盒准备无偏的fosmid文库，包括GELase酶和MaxPlax Lambda包装提取物▪ 通过拷贝数控制稳定克隆▪ 使用CopyControl HTP Fosmid库生产试剂盒优化高通量末端测序结果 |
| 组成成分： |
| ▪ End-It Enzyme Mix：-20℃▪ End-It 10X Buffer：-20℃▪ dNTP Mix (2.5 mM each) ：-20℃▪ ATP (10 mM) ：-20℃▪ Fast-Link DNA Ligase (2 U/µL) ：-20℃▪ End-It Enzyme Mix Fast-Link 10X Ligation Buffer：-20℃▪ GELase Enzyme Preparation (1 U/µL) ：-20℃▪ GELase 50X Buffer ：-20℃▪ Fosmid Control DNA (100 ng/µL) ：-20℃▪ pCC2FOS Fosmid Vector (0.5 µg/µL) ：-20℃▪ CopyControl Fosmid Autoinduction Solution (500X)：-20℃▪ Phage T1 Resistant EPI300 T1R Glycerol Stock ：-20℃▪ MaxPlax Lambda Packaging Extract ：-70℃▪ LE392MP Control Plating Strain Glycerol Stock ：-70℃▪ Ligated Lambda Control DNA (0.02 µg/µL) ：-70℃ |
| 数据： |
Figure 1. Production of a CopyControl™ Fosmid Library and subsequent inductin of clones to high-copy number.图1. CopyControl™Fosmid文库的生产以及随后的克隆诱导素到高拷贝数的复制。 |
技术参数 产品优点 - Prepare unbiased fosmid libraries with a complete kit, including GELase enzyme and MaxPlax Lambda Packaging Extracts - Stabilize clones with copy number control - Optimize high-throughput end-sequencing results with CopyControl HTP Fosmid Library Production Kit - 使用完整的试剂盒准备无偏的fosmid文库，包括GELase酶和MaxPlax Lambda包装提取物- 通过拷贝数控制稳定克隆- 使用CopyControl HTP Fosmid库生产试剂盒优化高通量末端测序结果
产品应用 - CopyControl Fosmid clones can be induced from single-copy to 10-20 copies per cell to improve DNA yields for sequencing, fingerprinting, subcloning, in vitro transcription, and other applications. - 可以将CopyControl Fosmid克隆从每细胞单拷贝复制到10-20拷贝，以提高DNA产量，用于测序，指纹分析，亚克隆，体外转录和其他应用。
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